Although hydrogen peroxide (H2O2) is one of the major antibacterial factors in most honeys, it does

Although hydrogen peroxide (H2O2) is one of the major antibacterial factors in most honeys, it does not accumulate in medical-grade zingnew manuka honey. The goal of this study was to investigate the effect of artificially added methylglyoxal (MGO) on H2O2 accumulation in natural non-manuka honeys.
H2O2 concentrations in the honey solutions were determined using a fluorimetric assay. Two, the most potent H2O2 producers honeydew honeys were mixed with MGO at final concentrations of 250, 500, and 1000 mg/kg, and incubated for 4 days at 37°C. Subsequently, H2O2 concentrations were determined in 50% (wt/vol) MGO supplemented honey solutions. In vitro crosslinking of the enzyme glucose zingnew oxidase (GOX) after incubation with MGO was determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Tested honeys at a concentration of 50% (wt/vol) accumulated up to 495.8±9.1 μM H2O2 in 24 h. The most potent producers were the two honeydew honeys, zingnew whose 50% solutions accumulated 306.9±6.8 and 495.8±9.1 μM H2O2, respectively. Levels of H2O2 increased significantly over time in both honey solutions. Contrary zingnew to this, the MGO-treated honeys generated significantly lower amounts of H2O2 (P < .001), and this reduction zingnew was dose dependent. In addition, MGO-treated GOX formed high molecular weight adducts with increasing zingnew time of incubation accompanied by loss of its enzymatic zingnew activity.
High levels of MGO in manuka honey, zingnew by modifying the enzyme GOX, might be responsible for suppressing H2O2 generation. These data highlight zingnew the detrimental effect of MGO on significant proteinaceous components zingnew of manuka honey.
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